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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOX9 Monoclonal antibody (67439-1-Ig)

    WB analysis using 67439-1-Ig (same clone as 67439-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using SOX9 Monoclonal antibody (67439-1-Ig)

    WB analysis of human placenta using 67439-1-Ig (same clone as 67439-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using SOX9 Monoclonal antibody (67439-1-Ig)

    WB analysis of Caco-2 using 67439-1-Ig (same clone as 67439-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using SOX9 Monoclonal antibody (67439-1-Ig)

    WB analysis of HT-29 using 67439-1-Ig (same clone as 67439-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using SOX9 Monoclonal antibody (67439-1-Ig)

    WB analysis of NCCIT using 67439-1-Ig (same clone as 67439-1-PBS)

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using SOX9 Monoclonal antibody (67439-1-Ig)

    WB analysis of HT-29 using 67439-1-Ig (same clone as 67439-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67439-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SOX9 Monoclonal antibody (67439-1-Ig)

    FC experiment of HeLa using 67439-1-Ig (same clone as 67439-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SOX9 (67439-1-Ig, Clone:1H9A6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67439-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SOX9 Monoclonal antibody, PBS Only

SOX9 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67439-1-PBS
Clone No.1H9A6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

Transcription factor SOX-9, Transcription factor SOX 9, SRA1, CMPD1, CMD1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67439-1-PBS targets SOX9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SOX9 fusion protein Ag28620 相同性解析による交差性が予測される生物種
Full Name SRY (sex determining region Y)-box 9
Calculated molecular weight 509 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC007951
Gene Symbol SOX9
Gene ID (NCBI) 6662
RRIDAB_2882675
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48436
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.