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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated SP1 Monoclonal antibody

SP1 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4G3H3

Cat no : CL594-66508

Synonyms

TSFP1, Specificity protein 1, Sp1 transcription factor, SP 1, 4G3H3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CoraLite®594-conjugated SP1 Monoclonal antibody (CL594-66508)

    IF Staining of human colon cancer using CL594-66508

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 SP1 antibody (CL594-66508, Clone: 4G3H3 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite®594-conjugated SP1 Monoclonal antibody (CL594-66508)

    IF Staining of HEK-293 using CL594-66508

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 SP1 antibody (CL594-66508, Clone: 4G3H3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite®594-conjugated SP1 Monoclonal antibody (CL594-66508)

    FC experiment of HEK-293 using CL594-66508

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SP1 (CL594-66508, Clone:4G3H3) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inHEK-293 cells, human colon cancer tissue
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66508 targets SP1 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SP1 fusion protein Ag16720 相同性解析による交差性が予測される生物種
Full Name Sp1 transcription factor
Calculated molecular weight 785 aa, 81 kDa
Observed molecular weight 95-105 kDa, 60-65 kDa
GenBank accession numberBC062539
Gene symbol SP1
Gene ID (NCBI) 6667
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].

Protocols

Product Specific Protocols
IF protocol for CL594 SP1 antibody CL594-66508Download protocol
FC protocol for CL594 SP1 antibody CL594-66508Download protocol
Standard Protocols
Click here to view our Standard Protocols