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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SP100 Recombinant antibody, PBS Only (Capture)

SP100 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241153F3

Cat no : 84012-4-PBS

Synonyms

Speckled 100 kDa, SP100 nuclear antigen, SP 100, Nuclear dot-associated Sp100 protein, Nuclear autoantigen Sp-100

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SP100 Recombinant antibody (84012-4-RR)

    WB analysis using 84012-4-RR (same clone as 84012-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84012-4-RR (SP100 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84012-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00964-1

    Cytometric bead array standard curve of MP00964-1

    Cytometric bead array standard curve of MP00964-1, SP100 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84012-4-PBS. Detection antibody: 84012-2-PBS. Standard: Ag35193. Range: 0.156-10 ng/mL.

  • Affinity and Kinetic Characterization of 84012-4-RR

    Affinity and Kinetic Characterization of 84012-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84012-4-RR against Human SP100 were performed. The affinity constant is 0.127 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84012-4-PBS targets SP100 as part of a matched antibody pair:

MP00964-1: 84012-4-PBS capture and 84012-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SP100 fusion protein Ag35193 相同性解析による交差性が予測される生物種
Full Name SP100 nuclear antigen
Calculated molecular weight 879 aa, 100 kDa
Observed molecular weight70-100 kDa
GenBank accession numberBC011562
Gene symbol SP100
Gene ID (NCBI) 6672
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains. Sp100 is an acidic protein that exerts a highly aberrant electrophoretic mobility in SDS-polyacrylamide gel electrophoresis.