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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SP100 Recombinant antibody

SP100 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241153F3

Cat no : 84012-4-RR

Synonyms

Speckled 100 kDa, SP100 nuclear antigen, SP 100, Nuclear dot-associated Sp100 protein, Nuclear autoantigen Sp-100

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SP100 Recombinant antibody (84012-4-RR)

    WB analysis using 84012-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84012-4-RR (SP100 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84012-4-RR

    Affinity and Kinetic Characterization of 84012-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84012-4-RR against Human SP100 were performed. The affinity constant is 0.127 nM.

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, Jurkat cells, MCF-7 cells, mouse spleen tissue, rat spleen tissue, mouse colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84012-4-RR is SP100 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SP100 fusion protein Ag35193 相同性解析による交差性が予測される生物種
Full Name SP100 nuclear antigen
Calculated molecular weight 879 aa, 100 kDa
Observed molecular weight70-100 kDa
GenBank accession numberBC011562
Gene symbol SP100
Gene ID (NCBI) 6672
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains. Sp100 is an acidic protein that exerts a highly aberrant electrophoretic mobility in SDS-polyacrylamide gel electrophoresis.

Protocols

Product Specific Protocols
WB protocol for SP100 antibody 84012-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols