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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SPAG5 Polyclonal antibody

SPAG5 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14726

Synonyms

sperm associated antigen 5, Mitotic spindle-associated protein p126, MAP126, hMAP126, Deepest

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated SPAG5 Polyclonal ant (CL488-14726)

    IF Staining of HeLa using CL488-14726

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 SPAG5 antibody (CL488-14726) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated SPAG5 Polyclonal ant (CL488-14726)

    FC experiment of HeLa using CL488-14726

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SPAG5 (CL488-14726) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14726 targets SPAG5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPAG5 fusion protein Ag6485 相同性解析による交差性が予測される生物種
Full Name sperm associated antigen 5
Calculated molecular weight 134 kDa
Observed molecular weight 135 kDa, 150 kDa
GenBank accession numberBC000322
Gene symbol SPAG5
Gene ID (NCBI) 10615
RRIDAB_2919100
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPAG5, also named as MAP126, Astrin and Deepest, is necessary for normal spindle formation during mitosis. It is highly expressed in testis. SPAG5 has different localization in somatic versus male germ cells suggesting the possibility of different function. This antibody is specific to SPAG5.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SPAG5 antibody CL488-14726Download protocol
FC protocol for CL Plus 488 SPAG5 antibody CL488-14726Download protocol
Standard Protocols
Click here to view our Standard Protocols