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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPAST Monoclonal antibody

SPAST Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1F5A7

Cat no : 67361-1-Ig

Synonyms

ADPSP, FSP2, KIAA1083, SPAST, Spastic paraplegia 4 protein, spastin, SPG4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPAST Monoclonal antibody (67361-1-Ig)

    WB analysis using 67361-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67361-1-Ig (SPAST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SPAST Monoclonal antibody (67361-1-Ig)

    IHC staining of mouse brain using 67361-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67361-1-Ig (SPAST antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SPAST Monoclonal antibody (67361-1-Ig)

    IHC staining of mouse brain using 67361-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67361-1-Ig (SPAST antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SPAST Monoclonal antibody (67361-1-Ig)

    IF Staining of mouse brain using 67361-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SPAST antibody (67361-1-Ig, Clone: 1F5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, NCCIT cells, HeLa cells, PC-3 cells, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67361-1-Ig targets SPAST in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SPAST fusion protein Ag18866 相同性解析による交差性が予測される生物種
Full Name spastin
Calculated molecular weight 616 aa, 67 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC150260
Gene symbol SPAST
Gene ID (NCBI) 6683
RRIDAB_2882613
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPAST encodes a microtubule-severing protein called spastin. By severing microtubules into small segments, SPAST participates in the regeneration of neurites. Mutations in the SPAST gene (previously known as SPG4) are the most common causes of hereditary spastic paraplegia (HSP-SPG4). Mammalian cells express at least two spastin isoforms, a less-abundant full-length form (M1-spastin, 68 kDa) and a more highly expressed isoform (M87-spastin, 60 kDa) that lacks the N-terminal 86 amino acids of the full-length protein. Also, there are two additional 64 kDa and 55 kDa isoforms due to alternative splicing of SPG4 exon 4. This antibody recognizes all these isoforms of spastin.

Protocols

Product Specific Protocols
WB protocol for SPAST antibody 67361-1-IgDownload protocol
IHC protocol for SPAST antibody 67361-1-IgDownload protocol
IF protocol for SPAST antibody 67361-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols