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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPAST Monoclonal antibody, PBS Only

SPAST Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F5A7

Cat no : 67361-1-PBS

Synonyms

ADPSP, FSP2, KIAA1083, SPAST, Spastic paraplegia 4 protein, spastin, SPG4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPAST Monoclonal antibody (67361-1-Ig)

    WB analysis using 67361-1-Ig (same clone as 67361-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67361-1-Ig (SPAST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SPAST Monoclonal antibody (67361-1-Ig)

    IHC staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67361-1-Ig (SPAST antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SPAST Monoclonal antibody (67361-1-Ig)

    IHC staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67361-1-Ig (SPAST antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SPAST Monoclonal antibody (67361-1-Ig)

    IF Staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SPAST antibody (67361-1-Ig, Clone: 1F5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67361-1-PBS targets SPAST in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SPAST fusion protein Ag18866 相同性解析による交差性が予測される生物種
Full Name spastin
Calculated molecular weight 616 aa, 67 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC150260
Gene symbol SPAST
Gene ID (NCBI) 6683
RRIDAB_2882613
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SPAST encodes a microtubule-severing protein called spastin. By severing microtubules into small segments, SPAST participates in the regeneration of neurites. Mutations in the SPAST gene (previously known as SPG4) are the most common causes of hereditary spastic paraplegia (HSP-SPG4). Mammalian cells express at least two spastin isoforms, a less-abundant full-length form (M1-spastin, 68 kDa) and a more highly expressed isoform (M87-spastin, 60 kDa) that lacks the N-terminal 86 amino acids of the full-length protein. Also, there are two additional 64 kDa and 55 kDa isoforms due to alternative splicing of SPG4 exon 4. This antibody recognizes all these isoforms of spastin.