Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
67361-1-PBS targets SPAST in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SPAST fusion protein Ag18866 相同性解析による交差性が予測される生物種 |
| Full Name | spastin |
| Calculated molecular weight | 616 aa, 67 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC150260 |
| Gene Symbol | SPAST |
| Gene ID (NCBI) | 6683 |
| RRID | AB_2882613 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UBP0 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
SPAST encodes a microtubule-severing protein called spastin. By severing microtubules into small segments, SPAST participates in the regeneration of neurites. Mutations in the SPAST gene (previously known as SPG4) are the most common causes of hereditary spastic paraplegia (HSP-SPG4). Mammalian cells express at least two spastin isoforms, a less-abundant full-length form (M1-spastin, 68 kDa) and a more highly expressed isoform (M87-spastin, 60 kDa) that lacks the N-terminal 86 amino acids of the full-length protein. Also, there are two additional 64 kDa and 55 kDa isoforms due to alternative splicing of SPG4 exon 4. This antibody recognizes all these isoforms of spastin.