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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse heart tissue using SPEG Polyclonal antibody (18716-1-AP)

    IHC staining of mouse heart using 18716-1-AP (same clone as 18716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18716-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SPEG Polyclonal antibody (18716-1-AP)

    IHC staining of mouse heart using 18716-1-AP (same clone as 18716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18716-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SPEG Polyclonal antibody (18716-1-AP)

    IHC staining of mouse skeletal muscle using 18716-1-AP (same clone as 18716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18716-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SPEG Polyclonal antibody (18716-1-AP)

    IHC staining of mouse skeletal muscle using 18716-1-AP (same clone as 18716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18716-1-PBS in a different storage buffer formulation.

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SPEG Polyclonal antibody, PBS Only

SPEG Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 18716-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18716-1-PBS targets SPEG in IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SPEG complex locus
Calculated molecular weight 354 kDa
GenBank accession numberNM_005876
Gene Symbol SPEG
Gene ID (NCBI) 10290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15772
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SPEG, also named as BPEG, APEG1 and KIAA1297, is highly similar to the corresponding rat and mouse proteins. Expression of SPEG is thought to serve as a marker for differentiated vascular smooth muscle cells which may have a role in regulating growth and differentiation of this cell type. SPEG is expressed in vascular smooth muscle cells and is down-regulated by vascular injury. Multiple transcripts are produced from the SPEG complex locus by alternative splicing and alternative promoter usage. The antibody recognizes the C-term of SPEG.