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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPHK1 Recombinant antibody (82609-1-RR)

    WB analysis using 82609-1-RR (same clone as 82609-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82609-1-RR (SPHK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82609-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPHK1 Recombinant antibody (82609-1-RR)

    IF Staining of HepG2 using 82609-1-RR (same clone as 82609-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPHK1 antibody (82609-1-RR, Clone: 2K12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82609-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82609-1-RR

    Affinity and Kinetic Characterization of 82609-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82609-1-RR against Human SPHK1 were performed. The affinity constant is below 1 pM.

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SPHK1 Recombinant antibody, PBS Only

SPHK1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82609-1-PBS
Clone No.2K12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Sphingosine kinase 1, SK1, EC:2.7.1.91, EC:2.3.1.-, Acetyltransferase SPHK1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82609-1-PBS targets SPHK1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SPHK1 fusion protein Ag1051 相同性解析による交差性が予測される生物種
Full Name sphingosine kinase 1
Calculated molecular weight 43 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC008040
Gene Symbol SPHK1
Gene ID (NCBI) 8877
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NYA1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).