Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, MCF-7 cells, NIH/3T3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82609-1-RR targets SPHK1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | SPHK1 fusion protein Ag1051 相同性解析による交差性が予測される生物種 |
| Full Name | sphingosine kinase 1 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC008040 |
| Gene Symbol | SPHK1 |
| Gene ID (NCBI) | 8877 |
| RRID | AB_3086491 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NYA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPHK1 antibody 82609-1-RR | Download protocol |
| IF protocol for SPHK1 antibody 82609-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |