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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using SPIB Polyclonal antibody (15768-1-AP)

    WB analysis of L02 using 15768-1-AP (same clone as 15768-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 15768-1-AP (SPIB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15768-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of L02 cells using SPIB Polyclonal antibody (15768-1-AP)

    IP experiment of L02 using 15768-1-AP (same clone as 15768-1-PBS)

    IP result of anti-SPIB (IP:15768-1-AP, 4ug; Detection:15768-1-AP 1:300) with L02 cells lysate 1800ug. This data was developed using the same antibody clone with 15768-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SPIB Polyclonal antibody (15768-1-AP)

    IHC staining of human liver cancer using 15768-1-AP (same clone as 15768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15768-1-AP (SPIB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15768-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SPIB Polyclonal antibody (15768-1-AP)

    IHC staining of human liver cancer using 15768-1-AP (same clone as 15768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15768-1-AP (SPIB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15768-1-PBS in a different storage buffer formulation.

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SPIB Polyclonal antibody, PBS Only

SPIB Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15768-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15768-1-PBS targets SPIB in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPIB fusion protein Ag8404 相同性解析による交差性が予測される生物種
Full Name Spi-B transcription factor (Spi-1/PU.1 related)
Calculated molecular weight 262 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC007921
Gene Symbol SPIB
Gene ID (NCBI) 6689
RRIDAB_2878182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01892
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.