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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    WB analysis of mouse brain using 10107-2-AP (same clone as 10107-2-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10107-2-AP (SPIN2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    IHC staining of human breast cancer using 10107-2-AP (same clone as 10107-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10107-2-AP (SPIN2B antibody) at dilution of 1:50 (under 25x lens). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    IHC staining of human colon using 10107-2-AP (same clone as 10107-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    IHC staining of human breast cancer using 10107-2-AP (same clone as 10107-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    IHC staining of human breast cancer using 10107-2-AP (same clone as 10107-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPIN2B Polyclonal antibody (10107-2-AP)

    IF Staining of HeLa using 10107-2-AP (same clone as 10107-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SPIN2B antibody (10107-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SPIN2B Polyclonal antibody (10107-2-AP)

    IF Staining of HEK-293 using 10107-2-AP (same clone as 10107-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SPIN2B antibody (10107-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

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SPIN2B Polyclonal antibody, PBS Only

SPIN2B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10107-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SPIN-2B, SPIN2_duplicate, SPIN-2, SPIN2, SPIN 2B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10107-2-PBS targets SPIN2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPIN2B fusion protein Ag0157 相同性解析による交差性が予測される生物種
Full Name spindlin family, member 2B
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC000044
Gene Symbol SPIN2B
Gene ID (NCBI) 474343
RRIDAB_2196120
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPZ2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.