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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using SPO11 Polyclonal antibody (15509-1-AP)

    WB analysis of mouse testis using 15509-1-AP (same clone as 15509-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15509-1-AP (SPO11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15509-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using SPO11 Polyclonal antibody (15509-1-AP)

    WB analysis of human testis using 15509-1-AP (same clone as 15509-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 15509-1-AP (SPO11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15509-1-PBS in a different storage buffer formulation.

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SPO11 Polyclonal antibody, PBS Only

SPO11 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 15509-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15509-1-PBS targets SPO11 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPO11 fusion protein Ag4340 相同性解析による交差性が予測される生物種
Full Name SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)
Calculated molecular weight 45 kDa
Observed molecular weight 55-70 kDa
GenBank accession numberBC033591
Gene Symbol SPO11
Gene ID (NCBI) 23626
RRIDAB_10639508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5K1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Spo11 is a protein used in a complex along with Mre11 and Rad50 during meiotic recombination. It is also involved in the creation of double stranded breaks in the DNA in the early stages of this process. Several transcript variants encoding different isoforms have been found for this gene.