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  • Featured Product
  • KD/KO Validated

SPTLC1 Monoclonal antibody, PBS Only

SPTLC1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D11D7

Cat no : 66899-1-PBS

Synonyms

Serine-palmitoyl-CoA transferase 1, LCB1, LCB 1, HSAN1, EC:2.3.1.50

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPTLC1 Monoclonal antibody (66899-1-Ig)

    WB analysis using 66899-1-Ig (same clone as 66899-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66899-1-Ig (SPTLC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SPTLC1 Monoclonal antibody (66899-1-Ig)

    WB analysis of HEK-293 using 66899-1-Ig (same clone as 66899-1-PBS)

    WB result of SPTLC1 antibody (66899-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using SPTLC1 Monoclonal antibody (66899-1-Ig)

    WB analysis of RAW 264.7 using 66899-1-Ig (same clone as 66899-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66899-1-Ig (SPTLC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SPTLC1 Monoclonal antibody (66899-1-Ig)

    IHC staining of human colon cancer using 66899-1-Ig (same clone as 66899-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SPTLC1 Monoclonal antibody (66899-1-Ig)

    IHC staining of human colon cancer using 66899-1-Ig (same clone as 66899-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66899-1-PBS targets SPTLC1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SPTLC1 fusion protein Ag7876 相同性解析による交差性が予測される生物種
Full Name serine palmitoyltransferase, long chain base subunit 1
Calculated molecular weight 53 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC007085
Gene symbol SPTLC1
Gene ID (NCBI) 10558
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1).