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SPTLC1 Monoclonal antibody

SPTLC1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D11D7

Cat no : 66899-1-Ig

Synonyms

Serine-palmitoyl-CoA transferase 1, LCB1, LCB 1, HSAN1, EC:2.3.1.50

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPTLC1 Monoclonal antibody (66899-1-Ig)

    WB analysis using 66899-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66899-1-Ig (SPTLC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SPTLC1 Monoclonal antibody (66899-1-Ig)

    WB analysis of HEK-293 using 66899-1-Ig

    WB result of SPTLC1 antibody (66899-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of RAW 264.7 cells using SPTLC1 Monoclonal antibody (66899-1-Ig)

    WB analysis of RAW 264.7 using 66899-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66899-1-Ig (SPTLC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SPTLC1 Monoclonal antibody (66899-1-Ig)

    IHC staining of human colon cancer using 66899-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SPTLC1 Monoclonal antibody (66899-1-Ig)

    IHC staining of human colon cancer using 66899-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, RAW 264.7 cells, HT-29 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66899-1-Ig targets SPTLC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SPTLC1 fusion protein Ag7876 相同性解析による交差性が予測される生物種
Full Name serine palmitoyltransferase, long chain base subunit 1
Calculated molecular weight 53 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC007085
Gene symbol SPTLC1
Gene ID (NCBI) 10558
RRIDAB_2882228
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1).

Protocols

Product Specific Protocols
WB protocol for SPTLC1 antibody 66899-1-IgDownload protocol
IHC protocol for SPTLC1 antibody 66899-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

EWS-FLI1 regulates and cooperates with core regulatory circuitry in Ewing sarcoma.

Authors - Xianping Shi
View Article