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Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66899 targets SPTLC1 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SPTLC1 fusion protein Ag7876 相同性解析による交差性が予測される生物種 |
| Full Name | serine palmitoyltransferase, long chain base subunit 1 |
| Calculated molecular weight | 53 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC007085 |
| Gene Symbol | SPTLC1 |
| Gene ID (NCBI) | 10558 |
| RRID | AB_2920053 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O15269 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 SPTLC1 antibody CL594-66899 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |