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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SRD5A1 Monoclonal antibody

SRD5A1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D5D8

Cat no : CL594-66329

Synonyms

S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated SRD5A1 Monoclonal antibody (CL594-66329)

    IF Staining of HeLa using CL594-66329

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66329 (SRD5A1 antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66329 targets SRD5A1 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SRD5A1 fusion protein Ag23351 相同性解析による交差性が予測される生物種
Full Name steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
Observed molecular weight 32 kDa
GenBank accession numberBC007033
Gene symbol SRD5A1
Gene ID (NCBI) 6715
RRIDAB_2883559
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRD5A1 is a minor component of the reductase activity in prostate although the gene was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant isozyme of steroid 5-alpha-reductase in the scalp and elsewhere in the skin.

Protocols

Product Specific Protocols
IF protocol for CL594 SRD5A1 antibody CL594-66329Download protocol
Standard Protocols
Click here to view our Standard Protocols