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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SRP54 Recombinant antibody, PBS Only

SRP54 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241125G1

Cat no : 84014-6-PBS

Synonyms

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 241125G1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRP54 Recombinant antibody (84014-6-RR)

    WB analysis using 84014-6-RR (same clone as 84014-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SRP54 Recombinant antibody (84014-6-RR)

    WB analysis using 84014-6-RR (same clone as 84014-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SRP54 Recombinant antibody (84014-6-RR)

    IHC staining of human placenta using 84014-6-RR (same clone as 84014-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84014-6-RR (SRP54 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84014-6-RR

    Affinity and Kinetic Characterization of 84014-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84014-6-RR against Human SRP54 were performed. The affinity constant is 4.24 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84014-6-PBS is SRP54 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SRP54 fusion protein Ag2327 相同性解析による交差性が予測される生物種
Full Name signal recognition particle 54kDa
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC003389
Gene symbol SRP54
Gene ID (NCBI) 6729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).