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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRP54 Recombinant antibody (84014-6-RR)

    WB analysis using 84014-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SRP54 Recombinant antibody (84014-6-RR)

    WB analysis using 84014-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SRP54 Recombinant antibody (84014-6-RR)

    IHC staining of human placenta using 84014-6-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84014-6-RR (SRP54 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SRP54 Recombinant antibody (84014-6-RR)

    IF Staining of HepG2 using 84014-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SRP54 antibody (84014-6-RR, Clone: 241125G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84014-6-RR

    Affinity and Kinetic Characterization of 84014-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84014-6-RR against Human SRP54 were performed. The affinity constant is 4.24 nM.

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SRP54 Recombinant antibody

SRP54 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84014-6-RR
Clone No.241125G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 241125G1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, RAW 264.7 cells, HEK-293 cells, MCF-7 cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84014-6-RR targets SRP54 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SRP54 fusion protein Ag2327 相同性解析による交差性が予測される生物種
Full Name signal recognition particle 54kDa
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC003389
Gene Symbol SRP54
Gene ID (NCBI) 6729
RRIDAB_3671584
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP61011
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).

Protocols

Product Specific Protocols
WB protocol for SRP54 antibody 84014-6-RRDownload protocol
IHC protocol for SRP54 antibody 84014-6-RRDownload protocol
IF protocol for SRP54 antibody 84014-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols