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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SRPX2 Monoclonal antibody

SRPX2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A1D7

Cat no : CL488-66266

Synonyms

BPP, CBPS, PMGX, RESDX, SRPUL, SRPX2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated SRPX2 Monoclonal ant (CL488-66266)

    IF Staining of A549 using CL488-66266

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 SRPX2 antibody (CL488-66266, Clone: 2A1D7 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66266 targets SRPX2 in IF/ICC applications and shows reactivity with human, pig, mouse samples.

Tested Reactivity human, pig, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SRPX2 fusion protein Ag8084 相同性解析による交差性が予測される生物種
Full Name sushi-repeat-containing protein, X-linked 2
Calculated molecular weight 465 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC020733
Gene symbol SRPX2
Gene ID (NCBI) 27286
RRIDAB_2883298
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRPX2 (sushi-repeat-containing protein, X-linked 2) is a secreted protein expressed in neurons of the human adult brain, including the rolandic area (PMID: 20874700). Firstly described as sushi-repeat protein up-regulated in leukemia (SPRUL) in leukemia cells with dysregulated expression at the transcriptional level, SRPX2 has been recently shown as a multifunctional protein. SRPX2 is a ligand for uPAR, the urokinase-type plasminogen activator (uPA) receptor (PMID: 18718938). It is involved in seizure disorders, angiogenesis and cellular adhesion (PMID: 22242148; 19667118; 16497722). The involvement of SRPX2 in disorders of language cortex suggests it has an important role in the perisylvian region critical for language and cognitive development (PMID: 16497722).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SRPX2 antibody CL488-66266Download protocol
Standard Protocols
Click here to view our Standard Protocols