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SRY Monoclonal antibody

SRY Monoclonal Antibody for WB, ELISA
Cat No. 67349-1-Ig
Clone No.2E9A4

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, ELISA

Testis-determining factor, Sex-determining region Y protein, sex determining region Y, 2E9A4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inLNCaP cells, DU 145 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67349-1-Ig targets SRY in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SRY fusion protein Ag12610 相同性解析による交差性が予測される生物種
Full Name sex determining region Y
Calculated molecular weight 204 aa, 24 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC074924
Gene Symbol SRY
Gene ID (NCBI) 6736
RRIDAB_2882607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ05066
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.

Protocols

Product Specific Protocols
WB protocol for SRY antibody 67349-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Recruitment of DNA to tumor-derived microvesicles.

Authors - James W Clancy
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