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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using SRY Monoclonal antibody (67349-1-Ig)

    WB analysis of LNCaP using 67349-1-Ig (same clone as 67349-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SRY Monoclonal antibody (67349-1-Ig)

    WB analysis using 67349-1-Ig (same clone as 67349-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SRY negative cell line HeLa cell line was served as negative control. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using SRY Monoclonal antibody (67349-1-Ig)

    WB analysis of DU 145 using 67349-1-Ig (same clone as 67349-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

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SRY Monoclonal antibody, PBS Only

SRY Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67349-1-PBS
Clone No.2E9A4

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

Testis-determining factor, Sex-determining region Y protein, sex determining region Y, 2E9A4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67349-1-PBS targets SRY in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SRY fusion protein Ag12610 相同性解析による交差性が予測される生物種
Full Name sex determining region Y
Calculated molecular weight 204 aa, 24 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC074924
Gene Symbol SRY
Gene ID (NCBI) 6736
RRIDAB_2882607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ05066
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.