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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SSSCA1 Monoclonal antibody

SSSCA1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D2E8

Cat no : CL488-68423

Synonyms

ZNRD2, Zinc ribbon domain-containing protein 2, Protein ZNRD2, p27, Autoantigen p27

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated SSSCA1 Monoclonal an (CL488-68423)

    IF Staining of HeLa using CL488-68423

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 SSSCA1 antibody (CL488-68423, Clone: 1D2E8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated SSSCA1 Monoclonal an (CL488-68423)

    FC experiment of HeLa using CL488-68423

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated SSSCA1 Monoclonal antibody (CL488-68423, Clone:1D2E8)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68423 targets SSSCA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SSSCA1 fusion protein Ag16783 相同性解析による交差性が予測される生物種
Full Name Sjogren syndrome/scleroderma autoantigen 1
Calculated molecular weight 199 aa, 21 kDa
Observed molecular weight23 kDa
GenBank accession numberBC014791
Gene symbol SSSCA1
Gene ID (NCBI) 10534
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about 23-27 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SSSCA1 antibody CL488-68423Download protocol
FC protocol for CL Plus 488 SSSCA1 antibody CL488-68423Download protocol
Standard Protocols
Click here to view our Standard Protocols