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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STAM Monoclonal antibody

STAM Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F5C2

Cat no : 68628-1-Ig

Synonyms

DKFZp686J2352, STAM, STAM 1, STAM1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STAM Monoclonal antibody (68628-1-Ig)

    WB analysis using 68628-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68628-1-Ig (STAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of RAW 264.7 cells using STAM Monoclonal antibody (68628-1-Ig)

    WB analysis of RAW 264.7 using 68628-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68628-1-Ig (STAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHCT 116 cells, RAW 264.7 cells, HeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68628-1-Ig targets STAM in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen STAM fusion protein Ag33192 相同性解析による交差性が予測される生物種
Full Name signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
Calculated molecular weight 59 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC030586
Gene symbol STAM
Gene ID (NCBI) 8027
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)

Protocols

Product Specific Protocols
WB protocol for STAM antibody 68628-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols