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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STAM Monoclonal antibody (68628-1-Ig)

    WB analysis using 68628-1-Ig (same clone as 68628-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68628-1-Ig (STAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68628-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using STAM Monoclonal antibody (68628-1-Ig)

    WB analysis of RAW 264.7 using 68628-1-Ig (same clone as 68628-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68628-1-Ig (STAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68628-1-PBS in a different storage buffer formulation.

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STAM Monoclonal antibody, PBS Only

STAM Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68628-1-PBS
Clone No.1F5C2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

DKFZp686J2352, STAM, STAM 1, STAM1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68628-1-PBS targets STAM in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen STAM fusion protein Ag33192 相同性解析による交差性が予測される生物種
Full Name signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
Calculated molecular weight 59 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC030586
Gene Symbol STAM
Gene ID (NCBI) 8027
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92783
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)