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CoraLite® Plus 488-conjugated STAM Polyclonal antibody
STAM Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-12434
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-12434 targets STAM in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | STAM fusion protein Ag3112 相同性解析による交差性が予測される生物種 |
| Full Name | signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 |
| Calculated molecular weight | 59 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC030586 |
| Gene symbol | STAM |
| Gene ID (NCBI) | 8027 |
| RRID | AB_2919053 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 STAM antibody CL488-12434 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
