STAR Monoclonal antibody
STAR Monoclonal Antibody for WB, IF-P, IF-Fro, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF-P, IF-Fro, ELISA
Conjugate
Unconjugated
CloneNo.
1G6E6
Cat no : 67130-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig adrenal gland tissue, human testis tissue, K-562 cells, rat adrenal gland tissue, rat testis tissue, THP-1 cells |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF-Fro detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
67130-1-Ig targets STAR in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | STAR fusion protein Ag28698 相同性解析による交差性が予測される生物種 |
| Full Name | steroidogenic acute regulatory protein |
| Calculated molecular weight | 285 aa, 32 kDa |
| Observed molecular weight | 28-32 kDa |
| GenBank accession number | BC010550 |
| Gene symbol | STAR |
| Gene ID (NCBI) | 6770 |
| RRID | AB_2882429 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STAR antibody 67130-1-Ig | Download protocol |
| IF protocol for STAR antibody 67130-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Endocrinol HE4 overexpression in mice leads to leydig cell hyperplasia and spermatogensis impairment: Pathological implications for oligospermia | ||
Toxicol Appl Pharmacol Reproductive effects of pubertal exposure to neonicotinoid thiacloprid in immature male mice | ||
Theriogenology Impacts of elevated temperature on morphology, oxidative stress levels, and testosterone synthesis in ex vivo cultured porcine testicular tissue |
