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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STAR Monoclonal antibody, PBS Only

STAR Monoclonal Antibody for WB, IF-P, IF-Fro, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, IF-Fro, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G6E6

Cat no : 67130-1-PBS

Synonyms

START domain-containing protein 1, STARD1, 1G6E6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of pig adrenal gland tissue using STAR Monoclonal antibody (67130-1-Ig)

    WB analysis of pig adrenal gland using 67130-1-Ig (same clone as 67130-1-PBS)

    pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat adrenal gland tissue using STAR Monoclonal antibody (67130-1-Ig)

    WB analysis of rat adrenal gland using 67130-1-Ig (same clone as 67130-1-PBS)

    rat adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using STAR Monoclonal antibody (67130-1-Ig)

    WB analysis of rat testis using 67130-1-Ig (same clone as 67130-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using STAR Monoclonal antibody (67130-1-Ig)

    WB analysis of K-562 using 67130-1-Ig (same clone as 67130-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using STAR Monoclonal antibody (67130-1-Ig)

    WB analysis of THP-1 using 67130-1-Ig (same clone as 67130-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using STAR Monoclonal antibody (67130-1-Ig)

    WB analysis of human testis using 67130-1-Ig (same clone as 67130-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using STAR Monoclonal antibody (67130-1-Ig)

    IF Staining of mouse testis using 67130-1-Ig (same clone as 67130-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (67130-1-Ig, Clone: 1G6E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using STAR Monoclonal antibody (67130-1-Ig)

    IF Staining of mouse testis using 67130-1-Ig (same clone as 67130-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (67130-1-Ig, Clone: 1G6E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using STAR Monoclonal antibody (67130-1-Ig)

    IF Staining of mouse testis using 67130-1-Ig (same clone as 67130-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 67130-1-Ig (STAR antibody) at dilution of 1:400, CoraLite®555 CL555-17178 (TNP1 antibody, orange) and CoraLite® Plus 488 CL488-13720 (BOULE antibody, green). This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67130-1-PBS targets STAR in WB, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STAR fusion protein Ag28698 相同性解析による交差性が予測される生物種
Full Name steroidogenic acute regulatory protein
Calculated molecular weight 285 aa, 32 kDa
Observed molecular weight 28-32 kDa
GenBank accession numberBC010550
Gene symbol STAR
Gene ID (NCBI) 6770
RRIDAB_2882429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue