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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STAT5B Monoclonal antibody

STAT5B Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D9B11

Cat no : 66427-1-Ig

Synonyms

STAT5A/B, STAT5, 1D9B11

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using STAT5B Monoclonal antibody (66427-1-Ig)

    WB analysis of Jurkat using 66427-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66427-1-Ig (STAT5B Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using STAT5B Monoclonal antibody (66427-1-Ig)

    WB analysis of HEK-293 using 66427-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66427-1-Ig (STAT5B Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STAT5B Monoclonal antibody (66427-1-Ig)

    IF Staining of HepG2 using 66427-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT5B antibody (66427-1-Ig, Clone: 1D9B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using STAT5B Monoclonal antibody (66427-1-Ig)

    IF Staining of HeLa using 66427-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT5B antibody (66427-1-Ig, Clone: 1D9B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using STAT5B Monoclonal antibody (66427-1-Ig)

    FC experiment of HeLa using 66427-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human STAT5B (66427-1-Ig, Clone:1D9B11) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG2a (KFA042), or 0.5 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66427-1-Ig targets STAT5B in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen STAT5B fusion protein Ag2709 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 5B
Calculated molecular weight 787 aa, 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC020868
Gene symbol STAT5B
Gene ID (NCBI) 6777
RRIDAB_2881798
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different GH. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Despite the 95% homology between the immunogen (Ag2709) and the STAT5A protein, our WB detection showed that this antibody does not recognize the STAT5A fusion protein (Ag19546).

Protocols

Product Specific Protocols
WB protocol for STAT5B antibody 66427-1-IgDownload protocol
IF protocol for STAT5B antibody 66427-1-IgDownload protocol
FC protocol for STAT5B antibody 66427-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols