CoraLite®594-conjugated STAT5B Monoclonal antibody
STAT5B Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1D9B11
Cat no : CL594-66427
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66427 targets STAT5B in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | STAT5B fusion protein Ag2709 相同性解析による交差性が予測される生物種 |
| Full Name | signal transducer and activator of transcription 5B |
| Calculated molecular weight | 787 aa, 90 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC020868 |
| Gene symbol | STAT5B |
| Gene ID (NCBI) | 6777 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different GH. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Despite the 95% homology between the immunogen (Ag2709) and the STAT5A protein, our WB detection showed that this antibody does not recognize the STAT5A fusion protein (Ag19546).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 STAT5B antibody CL594-66427 | Download protocol |
| FC protocol for CL594 STAT5B antibody CL594-66427 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
