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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STEAP4 Monoclonal antibody

STEAP4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A12G6

Cat no : 68465-1-Ig

Synonyms

DKFZp666D049, FLJ23153, Metalloreductase STEAP4, STAMP2, STEAP family member 4, STEAP4, TIARP, TNFAIP9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STEAP4 Monoclonal antibody (68465-1-Ig)

    WB analysis using 68465-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68465-1-Ig (STEAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Tested Applications

Positive WB detected inA549 cells, HaCaT cells, HeLa cells, MDA-MB-468 cells, SK-BR-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68465-1-Ig targets STEAP4 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STEAP4 fusion protein Ag30137 相同性解析による交差性が予測される生物種
Full Name STEAP family member 4
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC020600
Gene symbol STEAP4
Gene ID (NCBI) 79689
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STEAP4 (also called STAMP2), is a member of the STEAP family. Structurally, It is a 459 amino acid protein characterized by a molecular topology of six transmembrane domains. The cytoplasmic N-terminal domain of STEAP4 shows structural similarity with bacterial and archaeal FNO oxidoreductase and STEAP4 exhibits a strong iron reductase activity. Studies in mice and human suggest that STEAP4 maybe involved in adipocyte development and metabolism, and may contribute to the normal biology of the prostate cell, as well as prostate cancer progression.

Protocols

Product Specific Protocols
WB protocol for STEAP4 antibody 68465-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols