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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STEAP4 Monoclonal antibody (68465-1-Ig)

    WB analysis using 68465-1-Ig (same clone as 68465-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68465-1-Ig (STEAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68465-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STEAP4 Monoclonal antibody (68465-1-Ig)

    WB analysis using 68465-1-Ig (same clone as 68465-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68465-1-Ig (STEAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68465-1-PBS in a different storage buffer formulation.

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STEAP4 Monoclonal antibody, PBS Only

STEAP4 Monoclonal Antibody for WB, ELISA
Cat No. 68465-1-PBS
Clone No.2A12G6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

STEAP family member 4, STAMP2, Six-transmembrane epithelial antigen of prostate 4, Metalloreductase STEAP4, EC:1.16.1.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68465-1-PBS targets STEAP4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STEAP4 fusion protein Ag30137 相同性解析による交差性が予測される生物種
Full Name STEAP family member 4
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC020600
Gene Symbol STEAP4
Gene ID (NCBI) 79689
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ687X5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STEAP4 (also called STAMP2), is a member of the STEAP family. Structurally, It is a 459 amino acid protein characterized by a molecular topology of six transmembrane domains. The cytoplasmic N-terminal domain of STEAP4 shows structural similarity with bacterial and archaeal FNO oxidoreductase and STEAP4 exhibits a strong iron reductase activity. Studies in mice and human suggest that STEAP4 maybe involved in adipocyte development and metabolism, and may contribute to the normal biology of the prostate cell, as well as prostate cancer progression.