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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STIM1 Recombinant antibody

STIM1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B13

Cat no : 81156-1-RR

Synonyms

STRMK, stim-1, STIM 1, IMD10, GOK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STIM1 Recombinant antibody (81156-1-RR)

    WB analysis using 81156-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81156-1-RR (STIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using STIM1 Recombinant antibody (81156-1-RR)

    WB analysis using 81156-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81156-1-RR (STIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using STIM1 Recombinant antibody (81156-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 81156-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 81156-1-RR (STIM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STIM1 Recombinant antibody (81156-1-RR)

    IF Staining of HEK-293 using 81156-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using STIM1 antibody (81156-1-RR, Clone: 1B13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HepG2 cells, A549 cells, LNCaP cells, Jurkat cells, K-562 cells, Raji cells, RAW 263.7 cells, HSC-T6 cells, C6 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81156-1-RR targets STIM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STIM1 fusion protein Ag2140 相同性解析による交差性が予測される生物種
Full Name stromal interaction molecule 1
Calculated molecular weight 685 aa, 77 kDa
Observed molecular weight77 kDa
GenBank accession numberBC021300
Gene symbol STIM1
Gene ID (NCBI) 6786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.

Protocols

Product Specific Protocols
WB protocol for STIM1 antibody 81156-1-RRDownload protocol
IHC protocol for STIM1 antibody 81156-1-RRDownload protocol
IF protocol for STIM1 antibody 81156-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols