Validation Data Gallery
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Tested Applications
| Positive WB detected in | NIH/3T3 cells, RAW 264.7 cells, HSC-T6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-81156 targets STIM1 in WB applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | STIM1 fusion protein Ag2140 相同性解析による交差性が予測される生物種 |
| Full Name | stromal interaction molecule 1 |
| Calculated molecular weight | 685 aa, 77 kDa |
| Observed molecular weight | 77 kDa |
| GenBank accession number | BC021300 |
| Gene Symbol | STIM1 |
| Gene ID (NCBI) | 6786 |
| RRID | AB_3673724 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13586 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 STIM1 antibody CL750-81156 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |