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CoraLite® Plus 555-conjugated STIM1 Polyclonal antibody
STIM1 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 555 Fluorescent Dye
Cat no : CL555-11565
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | Jurkat cells |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL555-11565 targets STIM1 in FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | STIM1 fusion protein Ag2140 相同性解析による交差性が予測される生物種 |
Full Name | stromal interaction molecule 1 |
Calculated molecular weight | 685 aa, 77 kDa |
Observed molecular weight | 77-90 kDa |
GenBank accession number | BC021300 |
Gene symbol | STIM1 |
Gene ID (NCBI) | 6786 |
Conjugate | CoraLite® Plus 555 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 554 nm / 570 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.03% Proclin300, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 555 STIM1 antibody CL555-11565 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |