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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STK24 Monoclonal antibody

STK24 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2B4C1

Cat no : 68522-1-Ig

Synonyms

Serine/threonine-protein kinase 24 36 kDa subunit, Serine/threonine-protein kinase 24 12 kDa subunit, MST3, Mammalian STE20-like protein kinase 3, EC:2.7.11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STK24 Monoclonal antibody (68522-1-Ig)

    WB analysis using 68522-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68522-1-Ig (STK24 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using STK24 Monoclonal antibody (68522-1-Ig)

    IF Staining of rat brain using 68522-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using STK24 antibody (68522-1-Ig, Clone: 2B4C1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF-P detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68522-1-Ig targets STK24 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STK24 fusion protein Ag31526 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 24 (STE20 homolog, yeast)
Calculated molecular weight49KD
Observed molecular weight50 kDa
GenBank accession numberNM_003576
Gene symbol STK24
Gene ID (NCBI) 8428
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serine/threonine-protein kinase 24 (STK24), also named MST3, belonging to GCK subfamily of STE20-like kinases, acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation (PubMed: 10644707, PMID:12107159). STK24 and STK25 operate in the same cardiovascular development pathway with programmed cell death 10 (CCM3) (PMID: 20332113). It regulates axon outgrowth in forebrain neurons (PubMed: 17114295).

Protocols

Product Specific Protocols
WB protocol for STK24 antibody 68522-1-IgDownload protocol
IF protocol for STK24 antibody 68522-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols