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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STK24 Monoclonal antibody (68522-1-Ig)

    WB analysis using 68522-1-Ig (same clone as 68522-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68522-1-Ig (STK24 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68522-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using STK24 Monoclonal antibody (68522-1-Ig)

    IF Staining of rat brain using 68522-1-Ig (same clone as 68522-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using STK24 antibody (68522-1-Ig, Clone: 2B4C1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68522-1-PBS in a different storage buffer formulation.

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STK24 Monoclonal antibody, PBS Only

STK24 Monoclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 68522-1-PBS
Clone No.2B4C1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF-P, Indirect ELISA

Synonyms

Serine/threonine-protein kinase 24 36 kDa subunit, Serine/threonine-protein kinase 24 12 kDa subunit, MST3, Mammalian STE20-like protein kinase 3, EC:2.7.11.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68522-1-PBS targets STK24 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STK24 fusion protein Ag31526 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 24 (STE20 homolog, yeast)
Calculated molecular weight49KD
Observed molecular weight50 kDa
GenBank accession numberNM_003576
Gene Symbol STK24
Gene ID (NCBI) 8428
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y6E0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Serine/threonine-protein kinase 24 (STK24), also named MST3, belonging to GCK subfamily of STE20-like kinases, acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation (PubMed: 10644707, PMID:12107159). STK24 and STK25 operate in the same cardiovascular development pathway with programmed cell death 10 (CCM3) (PMID: 20332113). It regulates axon outgrowth in forebrain neurons (PubMed: 17114295).