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STK3 Monoclonal antibody
STK3 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse , Rat and More (1)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1C11A4
Cat no : 66637-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IF | See 1 publications below |
Product Information
66637-1-Ig targets STK3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.
Tested Reactivity | Human, Mouse , Rat |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | STK3 fusion protein Ag2741 相同性解析による交差性が予測される生物種 |
Full Name | serine/threonine kinase 3 (STE20 homolog, yeast) |
Calculated molecular weight | 491 aa, 56 kDa |
Observed molecular weight | 56-63 kDa |
GenBank accession number | BC010640 |
Gene symbol | STK3 |
Gene ID (NCBI) | 6788 |
RRID | AB_2881996 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for STK3 antibody 66637-1-Ig | Download protocol |
IF protocol for STK3 antibody 66637-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biochim Biophys Acta Gen Subj Extracellular matrix stiffness regulates degradation of MST2 via SCF βTrCP | ||
Oncogene Identification of a novel alternative splicing isoform of the Hippo kinase STK3/MST2 with impaired kinase and cell growth suppressing activities |