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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STK3 Monoclonal antibody (66637-1-Ig)

    WB analysis using 66637-1-Ig (same clone as 66637-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66637-1-Ig (STK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66637-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using STK3 Monoclonal antibody (66637-1-Ig)

    WB analysis of HeLa using 66637-1-Ig (same clone as 66637-1-PBS)

    WB result of STK3 antibody (66637-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK3 transfected HeLa cells. This data was developed using the same antibody clone with 66637-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using STK3 Monoclonal antibody (66637-1-Ig)

    IHC staining of mouse kidney using 66637-1-Ig (same clone as 66637-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66637-1-Ig (STK3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66637-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using STK3 Monoclonal antibody (66637-1-Ig)

    IHC staining of mouse kidney using 66637-1-Ig (same clone as 66637-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66637-1-Ig (STK3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66637-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using STK3 Monoclonal antibody (66637-1-Ig)

    IF Staining of NIH/3T3 using 66637-1-Ig (same clone as 66637-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using STK3 antibody (66637-1-Ig, Clone: 1C11A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66637-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

STK3 Monoclonal antibody, PBS Only

STK3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66637-1-PBS
Clone No.1C11A4

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MST2, MST 2, Mammalian STE20-like protein kinase 2, KRS1, EC:2.7.11.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66637-1-PBS targets STK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen STK3 fusion protein Ag2741 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 3 (STE20 homolog, yeast)
Calculated molecular weight 491 aa, 56 kDa
Observed molecular weight 56-63 kDa
GenBank accession numberBC010640
Gene Symbol STK3
Gene ID (NCBI) 6788
RRIDAB_2881996
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13188
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.