Validation Data Gallery
Tested Applications
Positive IF-P detected in | mouse brain tissue |
Positive IF/ICC detected in | NIH/3T3 cells |
Positive FC (Intra) detected in | Daudi cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-83081-2 targets STK39 in IF/ICC, IF-P applications and shows reactivity with human, rat samples.
Tested Reactivity | human, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | STK39 fusion protein Ag34503 相同性解析による交差性が予測される生物種 |
Full Name | serine threonine kinase 39 (STE20/SPS1 homolog, yeast) |
Calculated molecular weight | 59 kDa |
Observed molecular weight | 62 kDa |
GenBank accession number | BC166614 |
Gene Symbol | STK39 |
Gene ID (NCBI) | 27347 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9UEW8 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 STK39 antibody CL488-83081-2 | Download protocol |
FC protocol for CL Plus 488 STK39 antibody CL488-83081-2 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |