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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated STK39 Recombinant an (CL488-83081-2)

    IF Staining of mouse brain using CL488-83081-2

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 STK39 antibody (CL488-83081-2, Clone: 230382G9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite® Plus 488-conjugated STK39 Recombinant an (CL488-83081-2)

    IF Staining of NIH/3T3 using CL488-83081-2

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 488 STK39 antibody (CL488-83081-2, Clone: 230382G9 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Daudi cells using CoraLite® Plus 488-conjugated STK39 Recombinant an (CL488-83081-2)

    FC experiment of Daudi using CL488-83081-2

    1x10^6 Daudi cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Stk39 Recombinant Antibody (CL488-83081-2, Clone:230382G9)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated STK39 Recombinant antibody

STK39 Recombinant Antibody for IF/ICC, IF-P
Cat No. CL488-83081-2
Clone No.230382G9

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, IF-P

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inDaudi cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83081-2 targets STK39 in IF/ICC, IF-P applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STK39 fusion protein Ag34503 相同性解析による交差性が予測される生物種
Full Name serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
Calculated molecular weight59 kDa
Observed molecular weight62 kDa
GenBank accession numberBC166614
Gene Symbol STK39
Gene ID (NCBI) 27347
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UEW8
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 STK39 antibody CL488-83081-2Download protocol
FC protocol for CL Plus 488 STK39 antibody CL488-83081-2Download protocol
Standard Protocols
Click here to view our Standard Protocols