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STMN2 Monoclonal antibody

STMN2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F6C4

Cat no : 67204-1-Ig

Synonyms

SGC 10, SCGN10, SCGN 10, SCG10, SCG 10


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Tested Applications

Positive WB detected inPC-12 cells, fetal human brain tissue, mouse brain tissue, pig brain tissue, rat brain tissue, SH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

67204-1-Ig targets STMN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STMN2 fusion protein Ag28990 相同性解析による交差性が予測される生物種
Full Name stathmin-like 2
Calculated molecular weight 21 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC006302
Gene symbol STMN2
Gene ID (NCBI) 11075
RRIDAB_2882497
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STMN2 gene encodes a SCG10 (superior cervical ganglion-10) protein, a regulator of microtubule stability. When phosphorylated by MAPK8, SCG10 stabilizes microtubules and consequently controls neurite length in cortical neurons. SCG10 was early considered a neuron-specific growth-associated protein in Alzheimer's disease. In the developing brain, SCG10 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone.

Protocols

Product Specific Protocols
WB protocol for STMN2 antibody 67204-1-IgDownload protocol
IHC protocol for STMN2 antibody 67204-1-IgDownload protocol
IF protocol for STMN2 antibody 67204-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

A model of human neural networks reveals NPTX2 pathology in ALS and FTLD

Authors - Marian Hruska-Plochan