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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STMN2 Monoclonal antibody, PBS Only

STMN2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F6C4

Cat no : 67204-1-PBS

Synonyms

Protein SCG10, SCG10, SCGN10, SGC10, Stathmin 2, stathmin like 2, STMN2

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-12 cells using STMN2 Monoclonal antibody (67204-1-Ig)

    WB analysis of PC-12 using 67204-1-Ig (same clone as 67204-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using STMN2 Monoclonal antibody (67204-1-Ig)

    WB analysis of fetal human brain using 67204-1-Ig (same clone as 67204-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using STMN2 Monoclonal antibody (67204-1-Ig)

    WB analysis of pig brain using 67204-1-Ig (same clone as 67204-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using STMN2 Monoclonal antibody (67204-1-Ig)

    WB analysis of rat brain using 67204-1-Ig (same clone as 67204-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using STMN2 Monoclonal antibody (67204-1-Ig)

    WB analysis of mouse brain using 67204-1-Ig (same clone as 67204-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67204-1-Ig (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STMN2 Monoclonal antibody (67204-1-Ig)

    IHC staining of mouse brain using 67204-1-Ig (same clone as 67204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67204-1-Ig (STMN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STMN2 Monoclonal antibody (67204-1-Ig)

    IHC staining of mouse brain using 67204-1-Ig (same clone as 67204-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67204-1-Ig (STMN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using STMN2 Monoclonal antibody (67204-1-Ig)

    IF Staining of SH-SY5Y using 67204-1-Ig (same clone as 67204-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using STMN2 antibody (67204-1-Ig, Clone: 1F6C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67204-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67204-1-PBS targets STMN2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STMN2 fusion protein Ag28990 相同性解析による交差性が予測される生物種
Full Name stathmin-like 2
Calculated molecular weight 21 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC006302
Gene symbol STMN2
Gene ID (NCBI) 11075
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STMN2 gene encodes a SCG10 (superior cervical ganglion-10) protein, a regulator of microtubule stability. When phosphorylated by MAPK8, SCG10 stabilizes microtubules and consequently controls neurite length in cortical neurons. SCG10 was early considered a neuron-specific growth-associated protein in Alzheimer's disease. In the developing brain, SCG10 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone.