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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STMN2 Recombinant antibody, PBS Only

STMN2 Recombinant Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230110B12

Cat no : 82925-2-PBS

Synonyms

Protein SCG10, SCG10, SCGN10, SGC10, Stathmin 2, stathmin like 2, STMN2

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using STMN2 Recombinant antibody (82925-2-RR)

    WB analysis of SH-SY5Y using 82925-2-RR (same clone as 82925-2-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 82925-2-RR (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82925-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using STMN2 Recombinant antibody (82925-2-RR)

    IP experiment of SH-SY5Y using 82925-2-RR (same clone as 82925-2-PBS)

    IP result of anti-STMN2 (IP:82925-2-RR, 4ug; Detection:82925-2-RR 1:1500) with SH-SY5Y cells lysate 1240 ug. This data was developed using the same antibody clone with 82925-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STMN2 Recombinant antibody (82925-2-RR)

    IHC staining of mouse brain using 82925-2-RR (same clone as 82925-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-2-RR (STMN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82925-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STMN2 Recombinant antibody (82925-2-RR)

    IHC staining of mouse brain using 82925-2-RR (same clone as 82925-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-2-RR (STMN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82925-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using STMN2 Recombinant antibody (82925-2-RR)

    IF Staining of SH-SY5Y using 82925-2-RR (same clone as 82925-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using STMN2 antibody (82925-2-RR, Clone: 230110B12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 82925-2-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82925-2-PBS targets STMN2 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STMN2 fusion protein Ag0929 相同性解析による交差性が予測される生物種
Full Name stathmin-like 2
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC006302
Gene symbol STMN2
Gene ID (NCBI) 11075
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)