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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using STMN2 Recombinant antibody (82925-2-RR)

    WB analysis of SH-SY5Y using 82925-2-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 82925-2-RR (STMN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using STMN2 Recombinant antibody (82925-2-RR)

    IP experiment of SH-SY5Y using 82925-2-RR

    IP result of anti-STMN2 (IP:82925-2-RR, 4ug; Detection:82925-2-RR 1:1500) with SH-SY5Y cells lysate 1240 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STMN2 Recombinant antibody (82925-2-RR)

    IHC staining of mouse brain using 82925-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-2-RR (STMN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STMN2 Recombinant antibody (82925-2-RR)

    IHC staining of mouse brain using 82925-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-2-RR (STMN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STMN2 Recombinant antibody (82925-2-RR)

    IF Staining of mouse brain using 82925-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STMN2 antibody (82925-2-RR, Clone: 230110B12 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using STMN2 Recombinant antibody (82925-2-RR)

    IF Staining of SH-SY5Y using 82925-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using STMN2 antibody (82925-2-RR, Clone: 230110B12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using STMN2 Recombinant antibody (82925-2-RR)

    FC experiment of SH-SY5Y using 82925-2-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0 ug STMN2 Recombinant antibody (82925-2-RR, Clone:230110B12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82925-2-RR

    Affinity and Kinetic Characterization of 82925-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82925-2-RR against Human STMN2 were performed. The affinity constant is 1.48 nM.

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STMN2 Recombinant antibody

STMN2 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 82925-2-RR
Clone No.230110B12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Synonyms

SGC 10, SCGN10, SCGN 10, SCG10, SCG 10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.00 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82925-2-RR targets STMN2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STMN2 fusion protein Ag0929 相同性解析による交差性が予測される生物種
Full Name stathmin-like 2
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC006302
Gene Symbol STMN2
Gene ID (NCBI) 11075
RRIDAB_3670662
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ93045
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)

Protocols

Product Specific Protocols
WB protocol for STMN2 antibody 82925-2-RRDownload protocol
IHC protocol for STMN2 antibody 82925-2-RRDownload protocol
IF protocol for STMN2 antibody 82925-2-RRDownload protocol
IP protocol for STMN2 antibody 82925-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols