Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue, rabbit testis tissue, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
68071-1-Ig targets STRA8 in WB, IF, ELISA applications and shows reactivity with Human, rabbit, mouse samples.
| Tested Reactivity | Human, rabbit, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | STRA8 fusion protein Ag29680 相同性解析による交差性が予測される生物種 |
| Full Name | stimulated by retinoic acid gene 8 homolog (mouse) |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 43-60 kda |
| GenBank accession number | NM_182489 |
| Gene Symbol | STRA8 |
| Gene ID (NCBI) | 346673 |
| RRID | AB_2918812 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q7Z7C7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Stimulated by retinoic acid 8 (Stra8), one of genes induced by retinoic acid (RA), is required for the meiotic initiation of male spermatogenesis. Stimulated by Retinoic Acid Gene 8 (STRA8) protein has been proposed as the molecular effector of Retinoic Acid (RA) involved in meiosis-inductive activity. Stra8 is a 45-kDa vertebrate-specific gene, without significant homologous proteins, expressed in the cytoplasm and nuclei of germ cells between mitosis and meiosis and presents exclusively in the embryonic and postnatal gonads of mammals. (PMID: 30106128, PMID: 31253359)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STRA8 antibody 68071-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Environ Pollut Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling | ||
Food Funct Flaxseed oil attenuates PFOS-induced testicular damage by regulating RNA alternative splicing | ||
J Hazard Mater Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice |