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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using CoraLite® Plus 488-conjugated STRA8 Monoclonal ant (CL488-68071)

    IF Staining of NCCIT using CL488-68071

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using CoraLite® Plus 488 STRA8 antibody (CL488-68071, Clone: 1D7E3 ) at dilution of 1:200.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite® Plus 488-conjugated STRA8 Monoclonal antibody

STRA8 Monoclonal Antibody for IF/ICC
Cat No. CL488-68071
Clone No.1D7E3

Host / Isotype

Mouse / IgG1

Reactivity

Human, rabbit, mouse

Applications

IF/ICC

Synonyms

STRA8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inNCCIT cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68071 targets STRA8 in IF/ICC applications and shows reactivity with Human, rabbit, mouse samples.

Tested Reactivity Human, rabbit, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STRA8 fusion protein Ag29680 相同性解析による交差性が予測される生物種
Full Name stimulated by retinoic acid gene 8 homolog (mouse)
Calculated molecular weight 37 kDa
Observed molecular weight 43-60 kda
GenBank accession numberNM_182489
Gene Symbol STRA8
Gene ID (NCBI) 346673
RRIDAB_3084394
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7Z7C7
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stimulated by retinoic acid 8 (Stra8), one of genes induced by retinoic acid (RA), is required for the meiotic initiation of male spermatogenesis. Stimulated by Retinoic Acid Gene 8 (STRA8) protein has been proposed as the molecular effector of Retinoic Acid (RA) involved in meiosis-inductive activity. Stra8 is a 45-kDa vertebrate-specific gene, without significant homologous proteins, expressed in the cytoplasm and nuclei of germ cells between mitosis and meiosis and presents exclusively in the embryonic and postnatal gonads of mammals. (PMID: 30106128, PMID: 31253359)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 STRA8 antibody CL488-68071Download protocol
Standard Protocols
Click here to view our Standard Protocols