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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STXBP1 Polyclonal antibody (20562-1-AP)

    WB analysis using 20562-1-AP (same clone as 20562-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20562-1-AP (STXBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using STXBP1 Polyclonal antibody (20562-1-AP)

    IHC staining of human brain using 20562-1-AP (same clone as 20562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 20562-1-AP (STXBP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using STXBP1 Polyclonal antibody (20562-1-AP)

    IHC staining of human brain using 20562-1-AP (same clone as 20562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 20562-1-AP (STXBP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20562-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using STXBP1 Polyclonal antibody (20562-1-AP)

    IF Staining of HeLa using 20562-1-AP (same clone as 20562-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STXBP1 antibody (20562-1-AP) at dilution of 1:50 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20562-1-PBS in a different storage buffer formulation.

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STXBP1 Polyclonal antibody, PBS Only

STXBP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20562-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20562-1-PBS targets STXBP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name syntaxin binding protein 1
Calculated molecular weight 68 kDa
Observed molecular weight68 kDa
GenBank accession numberNM_003165
Gene Symbol STXBP1
Gene ID (NCBI) 6812
RRIDAB_10694824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61764
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions. The antibody is specific to STXBP1.