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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SURF1 Recombinant antibody

SURF1 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230210D5

Cat no : CL488-83052-7

Synonyms

Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1, 230210D5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated SURF1 Recombinant an (CL488-83052-7)

    FC experiment of HeLa using CL488-83052-7

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Surf1 Recombinant Antibody (CL488-83052-7, Clone:230210D5)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83052-7 targets SURF1 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SURF1 fusion protein Ag3959 相同性解析による交差性が予測される生物種
Full Name surfeit 1
Calculated molecular weight 33 kDa
Observed molecular weight30-31 kDa
GenBank accession numberBC028314
Gene symbol SURF1
Gene ID (NCBI) 6834
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 SURF1 antibody CL488-83052-7Download protocol
Standard Protocols
Click here to view our Standard Protocols