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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYAP1 Monoclonal antibody (68410-1-Ig)

    WB analysis using 68410-1-Ig (same clone as 68410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68410-1-Ig (SYAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68410-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of L02 cells using SYAP1 Monoclonal antibody (68410-1-Ig)

    IP experiment of L02 using 68410-1-Ig (same clone as 68410-1-PBS)

    IP result of anti-SYAP1 (IP:68410-1-Ig, 4ug; Detection:68410-1-Ig 1:2000) with L02 cells lysate 3080 ug. This data was developed using the same antibody clone with 68410-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SYAP1 Monoclonal antibody (68410-1-Ig)

    IF Staining of HepG2 using 68410-1-Ig (same clone as 68410-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SYAP1 antibody (68410-1-Ig, Clone: 2E12A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68410-1-PBS in a different storage buffer formulation.

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SYAP1 Monoclonal antibody, PBS Only

SYAP1 Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 68410-1-PBS
Clone No.2E12A5

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

DKFZp686K221, FLJ14495, FLJ44185, PRO3113, SYAP1, Synapse associated protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68410-1-PBS targets SYAP1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SYAP1 fusion protein Ag9626 相同性解析による交差性が予測される生物種
Full Name synapse associated protein 1, SAP47 homolog (Drosophila)
Calculated molecular weight 352 aa, 40 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC014657
Gene Symbol SYAP1
Gene ID (NCBI) 94056
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96A49
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.