Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | U2OS cells, A549 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IP detected in | L02 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68410-1-Ig targets SYAP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SYAP1 fusion protein Ag9626 相同性解析による交差性が予測される生物種 |
| Full Name | synapse associated protein 1, SAP47 homolog (Drosophila) |
| Calculated molecular weight | 352 aa, 40 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC014657 |
| Gene Symbol | SYAP1 |
| Gene ID (NCBI) | 94056 |
| RRID | AB_3085127 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96A49 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SYAP1 antibody 68410-1-Ig | Download protocol |
| IF protocol for SYAP1 antibody 68410-1-Ig | Download protocol |
| IP protocol for SYAP1 antibody 68410-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |